Forensic Microbiological Analysis for Vaginal Fluid Identification Using Multiplex Real-Time PCR System

mRNA Profiling: Body Fluid Identification Using Multiplex Real-Time PCR

Messenger Rna Profiling: Body Identification Using Multiplex Real-time Pcr

Conventional methods of body fluid identification use labor-intensive, technologically diverse techniques that are performed in a series, not parallel, manner and are costly in terms of time and sample. Furthermore, for some frequently encountered body fluids no confirmatory technique exists. There is no definitive test, for example, for the presence of saliva or vaginal secretions. In seeking ...

Real-time multiplex PCR assays.

The ability to multiplex PCR by probe color and melting temperature (T(m)) greatly expands the power of real-time analysis. Simple hybridization probes with only a single fluorescent dye can be used for quantification and allele typing. Different probes are labeled with dyes that have unique emission spectra. Spectral data are collected with discrete optics or dispersed onto an array for detect...

Microbiological surveillance of a bovine raw milk farm through multiplex real-time PCR.

Raw milk is increasingly appreciated by consumers but can be contaminated by a variety of zoonotic pathogens. Therefore, preventive measures, such as on-farm hazard analysis critical control point (HACCP) programs, must be applied to protect consumers. The aim of the present study was the comparison of a multiplex real-time polymerase chain reaction (PCR) assay with a culture-based approach in ...

Key words: herpes simplex virus, real-time PCR, multiplex real-time PCR assay

要 旨 単 純 ヘ ル ペ ス ウ イ ル ス1型(HSV-1)お よ び2型(HSV-2)の 遺 伝 子 を 同時 に検 出 す るmultiplex real-time PCR法 を 開発 し た. HSV-1お よびHSV-2のgpD領 域 を標 的 と し,プ ラ イ マ ー お よ びTaqMan蛍 光 プ ロ ー ブ を 独 自 に 設 計 し た.検 出感 度 は15copies/tubeで,HSV-1お よ びHSV-2と も15か ら1.5×106 copies/tubeの 範 囲 で 定 量 が 可 能 で あ っ た.real-time PCR法 はPCR法 よ り も103倍 感 度 が 高 く,他 の ヘ ルペ ス属(CMV, EBV, HHV6, HHV-7お よ びVZV) DNAは 検 出 され なか っ た. 以 上 の結 果 か ら,今 回 開 発...